The applications in healing periodontal defects by regenerating new

The small-molecule
compounds on periodontal ligament stem cells (PDLSCs) are of great interest for the study of tissue
maintenance and regeneration as the PDLSCs is one of the tissues with the
highest turnover rate and remodeling capacity in the human body. In addition,
Some study reported that small-molecule compounds in Extracellular matrix (ECM) could induce development of
PDLSC-like cells form iPS cells.

The first report to outline the production of PDLSCs from
iPS cells to iPS-NC-PDL cells acquired the proliferative and
differentiative properties of PDL cells and expressed markers typical of Mesenchymal
stem cell (MSCs), revealed that ECM from PDL cells was
the most efficient of iPS-NC-PDL cells, with a characteristics similar to that
of primary PDLSCs. Although Collagen type I (COL1) and Periostin (POSTN) are
the major ECM proteins in PDL tissue, they failed to increase the expression of
PDL-related markers and insufficient to drive PDLSC-like differentiation of
iPS-NC cells. Moreover fibronectin and laminin showed some increase, it was not
as efficient as the PDL ECM. (Hamano et al.,
2017)

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The number of other small-molecule
compounds in ECM have potential in differentiation of PDLSCs, Some of
studies reported that ECM growth factor such
as Connective tissue growth
factor (CTGF) and Fibroblast growth factor 2 (FGF2) induce PDLSCs to differentiate towards a PDL-like tissue in a collagen gel microenvironment and might thus be suitable for
periodontal regeneration therapy. In addition, RGD-modified hydrogels maintained
cell viability, attachment and increased the mineralization of the encapsulated
DFCs, and thus could have potential applications in healing periodontal defects
by regenerating new bone and cementum.Therefore, they would be interesting to know as the following aspects: (1) Role
small-molecule compounds derivative in ECM produced by
cultured human PDL cells in differentiation of iPS cells to PDLSCs; (2) Identification of small molecule compounds in ECM are necessary for inducing PDL cells from iPS cells.