LIPASE and the aqueous phase where enzyme remains break.

                                                     

 

        LIPASE enzymes catalyze the hydrolysis of
triglycerides to glycerol. Lipases are water soluble enzyme. Lipases belong to
the class of serine hydrolases, and produced by animal plants. Lipases catalyze
the hydrolysis of ester bonds down at the surface between insoluble phase and
the aqueous phase where enzyme remains break. Many microorganisms secrete
lipases for the digestion of lipid. Fungal lipases represent an important group
of biotechnologically important enzymes because of the versatility of its properties
which make it very attractive for industrial applications. Lipases are
increasingly produced recombinant DNA technology. Fungal lipase get observation
in the industries due to specificity and stability under physical and chemical
conditions. They can be extracted easily. 
Lipase has ability to perform bio transformation has made them increase
in the food, detergent, pharmaceutical industries, medicine found in soils
contaminated with oils, wastes of vegetable oils, dairy product industries,
seeds, maturation of cheese, synthesis of aromas these are the application in
food industry of lipases.  Industrial
application of lipase is fungal, mold or bacterial origin are extracellular and
can be produced in economically feasible systems. Fungi are the best source of
enzymes as they secrete abundant amount of enzymes extracellularly, thereby
simple extractions from different fermentation media. Lipase are vital enzymes
stability in both aqueous and non-aqueous media, perform catalytic reaction.
Lipase obtained different sources may have different properties. A real lipase
converts emulsified esters of glycerol and long-chain fatty acids into polar
lipid. Lipase reactions take place between the aqueous and oil phases due to
opposite orientation of poles between the enzymes and their substrates. Lipase
can be present in plants, microorganisms. Lipase plant has been obtained from
rice bran, Jatropha seeds and palm oils. Production of lipase based on isolate.
Lipase from the aspergillus niger 6516 using solid state fermentation on
Jtropha seed cake as the medium and find the 
lipase ativity, temperature, ph. Lipasde is only valid enzyme which is
used in synthesis biodiesel. Lipase is the best applicant, it can produce
microbes, microbial engineering research development to very elevated lipase.
Aspergillus niger classified as (GRAS Generally Regarded as Safe) by the (FDA
Food and Drug Administration united state of America).

 

In biotechnology, aspergillus niger is one of the important
species. Lipases from aspergillus species have been studied for industrial
application food, medicine, detergent. Industrial processes are run above above
45 °C, enzyme are thermostable at 50 °C. Thermostable enzymes have advantages
in industrial processes, increase reaction rate with increase of temperature
which turn to decrease viscosity of liquid.

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 Lipases from
aspergillus niger with unique properties, high tolerance to organic solvents,
and food industrial applications. The applications of aspergillus Niger lipase
in the food industry is enzyme-assisted enrichment for n-3 polyunsaturated
fatty acids (PUFA) from fish oil. PUFA, especially eicosapentaenoic acid (EPA)
and docosahexaenoic acid (DHA), have beneficial effects on coronary heart
disease, cancer and nervous system development

Microbial lipase have great interest from chemical and
pharmaceutical industries due to application in both hydrolysis and synthesis
reaction. The use of lipase for the synthesis of compounds esterification and
tranesterfication.

The biotechnological interest in lipase enzymes is
biotransformation. Aspergillus niger yeast, the produced lipase used in
industry, 298 amino acids, 4 tryptophan’s. Aspergillus niger shows position
towards 1 – and 3- positions of glycerol moiety, substrate for medium chain
length of fatty acids, considered (GRAS) by the food and drug administration of
U.S make it food addictive in the food industry. Aspergillus niger has been
registered to the acetylation and produced different strains isolated from oil
staining soil samples and organisms was identified as A.niger F044.

Lipases was obtained from new sources in order to determine
their application, characterize the lipases produced by Aspergillus flavus and
Aspergillus Niger through submerged and solid-state fermentation, according to
the optimum temperature and pH, and to determine the stability of enzymes in correlation
to temperature and ph. Production, purification and characterization of a
lipase from a fungal isolate of Aspergillus terreus grown on agro substrates
under both submerged fermentation (SmF) and SSF conditions. Fungal lipase
production, process operation, purification, and its industrial applications.

Enzymes show us a wide range of applications in different
industries whether it may be food, textile, medicine, dairy, or any other.
Modern biotechnology and protein engineering have the ability to introduce or
modify the ability of the genes. Microbial enzymes and use in different
industries along with the biotechnological perspectives of its future
development.

 Lipase activity and
production level changes according to microorganisms with conditions in the
cultivation. Solid state fermentation with mutant aspergillus niger has
efficiency to produce enzymes. SSF microbial culture are closer to natural
habitat and their activity is increased. SSF is basically the growth of
microorganism on solid matter. Absence of water. Many disadvantages of SSF are
low efficiency costs, use of agricultural waste products as substrate. Agro
industrial are considered best substrate for SSF processes and production of
enzyme they are cheap, abundant, rice husk, wheat bran also used in SSF
processes.

Aspergillus niger is best lipase producers used in dairy
industry. New lipase producers are variable and important due to industrial
applications which need of lipolytic enzymes which have desired properties.

       Lipases was obtained
from new sources in order to determine their application, characterize the
lipases produced by Aspergillus flavus and Aspergillus Niger through submerged
and solid-state fermentation, according to the optimum temperature and pH, and
to determine the stability of enzymes in correlation to temperature and ph. Production,
purification and characterization of a lipase from a fungal isolate of
Aspergillus terreus grown on agro substrates under both submerged fermentation
(SmF) and SSF conditions. Fungal lipase production, process operation,
purification, and its industrial applications.

Enzymes show us a wide range of applications in different
industries whether it may be food, textile, medicine, dairy, or any other.
Modern biotechnology and protein engineering have the ability to introduce or
modify the ability of the genes. Microbial enzymes and use in different
industries along with the biotechnological perspectives of its future
development.