Cutaneous and this gene is a leishmania glycoprotein of

Cutaneous leishmaniasis is one of the major diseases in tropical
and subtropical areas. It is caused by parasites of Leishmania tropica and
Leishmania major. It is a health problem in 88 countries, and because of the
importance of controlling and preventing its spread, it is one of the top
priorities of the international community, including the World Health
Organization. Today, chemotherapy, especially the use of five-component
antimony compounds, such as glucantime, is used more than other methods. The
use of glucantime in patients with cutaneous leishmaniasis causes kidney,
liver, heart, and blood disorders. Studies conducted in different parts of the
world show drug resistance in some people with leishmaniasis compared to
glucantime. Studies conducted in different parts of the world show drug
resistance in some people with leishmaniasis compared to glucantime. The drug
resistance to leishmaniasis may be normal, which can be caused by reduced
ability of the host’s immune system, drug defect, and inadequate treatment or
due to unusual consumption and an increase in the duration of glucantime
consumption that causes mutations in the genomic leishmaniasis, which in both
cases causes no response to treatment. In the drug resistance, it has been shown
to increase the production of glycoprotein, which is involved in the
transmission of ABC gene, and this gene is a leishmania glycoprotein of type
MDR1 located on chromosome 34. It is an ATP-dependent delivery pump that can
reduce the drug’s accumulation in resistant cells and often increase drug
resistance. In order to identify gene sequence variations, due to the accuracy,
cost and time spent, the HRM (High resolution metion)
method seems to be a suitable and powerful tool. HRM is a new and homogeneous
method after PCR amplification performed in a sealed tube. This method of
genetic variation analysis (SNPs, mutations and methylation) measures PCR
products based on the amount of fluorescence reduction during the process of
thermal melting  slope of the DNA due to
color exhaustion and HRM products can be sequenced without any additional
replication and purification Based on reports of drug resistance caused by
glucantime in patients with cutaneous leishmaniasis, this study was conducted
to determine and diagnose mutations in the MDR1 drug resistance gene in
paraffin tissue samples of leishmaniasis.