Application· Detect gene expression of mRNAs ofa particular cell at different times, measure gene expression levels· Diagnose pathogenic, geneticdiseases · Predict prognosis, treatmentoutcome · Proteomic analysis· Comparative genomics to analyzegenomic alterations, detecting polymorphisms and mutations · Transcription factor bindinganalysis: determine the binding sites of transcription factor· Classification of tumors· Drug discovery Laser capturemicrodissection (LCM) This technique allowisolation of a target sample from a heterogeneous mixture, provide a moreefficient and accurate results for downstream microgenomics applications suchas next-generation sequencing, Sanger sequencing, PCR and proteomics. Thistechnique do not disturb the morphology of the chosen cells thus can directlyvisualized after microdissection. LCM can minimize contamination and to uncoveranswers that might be missed using a nontargeted sample due to the small sizeor homogenized nature of the sample. PrincipleProcurement of pure populationsof cells from tissue sections in one shot under direct microscopicvisualization which connected to LCM, then laser transfer for excising thecells and capturing into a collection tube then further evaluate. First, thehistological preparation on a glass slide are overlain by a thermoplastic filmandis melted by low energy infrared laser pulse.
The melted membrane forms acomposite with the selected tissue area which can be removed by lifting of themembrane and directly immerged in extraction buffer. Application· Genetic analysis: Facilitate comparative studies betweennormal, premalignant and malignant cells from the same individual· Crucial for preserving genomic molecules. · Allow the establishment of “genetic fingerprints” ofspecific pathological lesions especially malignant neoplasms· Study of subpopulations of cells of a specificimmunophenotype· Help in establishing individualized treatments tailored tothe molecular profile of a tumor